Research Publications
Tiedje, J.M., and T.O. Stevens. 1988. The ecology of an anaerobic dechlorinating consortium.
in G.S. Omen (ed.).Environmental Biotechnology: Reducing risks from environmental chemi-
cals through biotechnology. Plenum Press,
 New York. pp. 3-14.
Stevens, T.O., T.G. Linkfield, and J.M. Tiedje. 1988. Physiological characterization of strain
DCB-1, a unique dehalogenating sulfidogenic bacterium. Appl. Environ. Microbiol.
Stevens, T.O., and J.M. Tiedje. 1988. Carbon dioxide fixation and mixotrophic metabolism by
strain DCB-1, a dehalogenating anaerobic bacterium. Appl. Environ. Microbiol. 54(12):2944-
Stevens, T.O., R.L. Crawford and D.L. Crawford. 1990. Biodegradation of dinoseb (2-sec-
butyl-4,6-dinitrophenol) in several Idaho soils varying in dinoseb exposure history. Appl. Envi-
ron. Microbiol. 56(1) 133-139.
Stevens, T.O., R.L. Crawford, and D.L. Crawford. 1991. Selection and isolation of bacteria
capable of degrading dinoseb (2-sec-butyl-4,6-dinitrophenol). Biodegradation 2:1-13.
Fredrickson, J.K., F.J. Brockman, S.W. Li, D.J. Workman, T.O. Stevens. 1991. Isolation and char-
acterization of a subsurface bacterium capable of growth on toluene, naphthalene, and
other aromatic compounds. Appl. Environ. Microbiol. 57:796-803.
Kaake, R.H., D.J. Roberts, T.O. Stevens, R.L. Crawford, D.L. Crawford. 1992. Bioremediation of
soils contaminated with the herbicide 2-sec-butyl-4,6-dinitrophenol (Dinoseb). Appl. Environ.
Microbiol. 58:1683-1689.
Stevens, T.O. and J.K. Fredrickson. 1992. Potential for microbial gas generation in high-level
radioactive waste storage tanks. Report # DSTSS91:0007. in Bryan, S.A., et. al., Slurry growth,
gas retention, and flammable gas generation by Hanford radioactive waste tanks: Synthetic
waste studies, FY 1991. PNL-8169 UC-721.
Stevens, T.O., J.P. McKinley, and J.K. Fredrickson. 1993. Bacteria associated with deep, alka-
line, anaerobic groundwaters in SE Washington. Microbial Ecology. 25:35-50.
Fruchter, J.S., F.A. Spane, J.E. Amonette, J.K. Fredrickson, C.R. Cole, J.C. Templeton, T.O. Ste-
vens, D.J. Holford, L.E. Eary, J.M. Zachara, B.N. Bjornstad, G.D. Black, V.R. Vermeul, C.S. Sim-
mons. 1994. Interim report: Manipulation of natural subsurface processes: Field research and
validation. PNL-10123 UC-402,802.
Bjornstad, B.N.,, J.P. McKinley, T.O. Stevens , S.A. Rawson, J.K. Fredrickson, P.E. Long. 1994.
Generation of hydrogen gas as a result of drilling within the saturated zone. Groundwater
Monitoring and Remediation Fall 1994. 140-147.
Boone, D.R., Y. Liu, Z-J Zhao, D.L. Balkwill, G.R. Drake, T.O. Stevens, and H.C. Aldrich. 1995.
Bacillus infernus sp. nov., an Fe(III)-and Mn(IV)-reducing anaerobe from a deep terrestrial
subsurface. Int. J. System. Bacteriol. 45:441-448.
Stevens, T.O. 1995. Optimization of media for enumeration and isolation of aerobic het-
erotrophic bacteria from the deep terrestrial subsurface. Journal of Microbiological Methods.
Stevens, T.O. and B.S. Holbert. 1995. Variability and density-dependence of bacteria in terres-
trial subsurface samples: implications for enumeration. Journal of Microbiological Methods.
Stevens, T.O. and J.P. McKinley. 1995. Lithoautotrophic microbial ecosystems in deep basalt
aquifers. Science 270:450-454.
Stevens, T.O. 1997. Subsurface microbiology and the evolution of the biosphere. in Amy, P.S.
and Haldeman, D.L. eds The Microbiology of the Terrestrial Subsurface. pp. 205-223. CRC Press.
Boca Raton.
McKinley, J.P., T.O. Stevens, J.K. Fredrickson, J.M. Zachara, F.C. Colwell, K.B. Wagnon, S.C. Smith,
S.A. Rawson, and B.N. Bjornstad. 1997. Biogeochemistry of anaerobic lacustrine and paleosol
sediments within an aerobic unconfined aquifer. Geomicrobiol. J. 14:23-39.
Space Studies Board, National Research Council. 1997. Mars Sample Return: Issues and Recom-
mendations. Nat. Acad.
Press. Washington, D.C. ( also at : )
Stevens, T.O. 1997. Lithoautotrophy in the subsurface. FEMS Microbiol. Rev. 20:327-337.
Stevens, T.O. 1997. Subsurface lithoautotrophic microbial ecosystems (SLMEs) in igneous rocks:
Prospects for detection. Instruments, Methods, and Missions for the Investigation of Extraterres-
trial Microorganisms. Soc. Photo-Opitcal  Instr. Eng. Proc. 3111:358-365.
Onstott, T.C., T.J.Phelps, F.S. Colwell, D. Ringelberg, D.C. White, D.R. Boone, J.P. McKinley, T.O.
Stevens, D.L. Balkwill, and T. Griffin. 1998. The subsurface environment of deep-dwelling bacte-
ria in the Triassic age Taylorsville Basin. Geomicrobiol. J. 15:353-385
Thomas-Keprta, K., D. McKay, S.J. Wentworth, T.O. Stevens, A.E. Taunton, C.C. Allen, E.K.Gibson,
Jr., and C.S. Romanek.  1998. Bacterial mineralization patterns in basaltic environments: impli-
cations for possible life in Martian meteorite ALH84001. Geology 26:1031-1034.
McKinley, J.P., T.O. Stevens, and F. Westall. 2000. Deep subsurface microfossils and
paeloenvironments within the Columbia River Basalt group. Geomicrobiol. J 17: 43-54.
Stevens T.O. and J.P. McKinley. 2000. Abiotic controls on H2 production from basalt-water reac-
tions and implications  for aquifer biogeochemistry. Environ. Sci. Technol. 34:826-831.
Stevens, T.O. 2002. The deep subsurface biosphere. In J.T. Staley and A.-L. Reysenbach (Eds.)
Biodiversity of Microbial Life. Pp 439-474. Wiley-Liss, New York.
Stevens, T.O. and Dunagan, S.E. 2005. Methods for aseptic sampling of hard-rock cores. Submit-
Stevens, T.O., Ramils, R., Fernandez-Remolar, D., and Stoker, C.R. 2005. A subsurface ecosystem
fed by a volcanically hosted massive sulfide deposit: Peña del Hierro, Rio Tinto, Spain. Submit-
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